Zusammenfassung
Zur Diagnostik einer unklaren intraokularen Entzündung kann die Kammerwasseranalyse
hilfreich sein. Labormethoden wie die Antikörperbestimmung mittels mikroELISA, Polymerasekettenreaktion
(PCR) oder die Zytokinbestimmung erlauben einen Informationsgewinn, der diagnostisch
entscheidend sein kann. Voraussetzung für Qualität und Aussagekraft der Untersuchung
sind korrekte Indikationsstellung und adäquate Verarbeitung des geringen Probenmaterials.
Vor diesem Hintergrund widmet sich dieser Beitrag der Gewinnung und Aufarbeitung von
Kammerwasser für diagnostische Zwecke bei intraokularer Entzündung.
Abstract
For the diagnosis of unclear intraocular inflammation, the collection of aqueous humor
can provide substantial information. Specific procedures including microELISA, polymerase
chain reaction (PCR) and cytokine analysis may provide essential diagnostic information.
The correct indication and an adequate processing of ocular samples determine the
quality and reliability of the laboratory results. This article describes the different
considerations and processes that are important to ensure optimal results for these
different diagnostic laboratory procedures.
Schlüsselwörter
Kammerwasser - intraokulare Entzündungen - Mikrobiologie/Zytologie - Retina - Diagnostik
- Uveitis
Key words
aqueous humor - Intraocular Inflammation - microbiology/cytology - Retina - diagnostic
- uveitis
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1 Teile des Beitrags stehen in Verbindung zum BMBF-Projekt: Förderung zur Toxoplasmose
beim Menschen und Tieren in Deutschland: Pathogenese, Risikofaktoren und Kontrolle
– TOXONET 02.
Prof. Dr. Uwe Pleyer
Universitäts-Augenklinik, Charité Campus Virchow-Klinik
Augustenburger Platz 1
13353 Berlin
Phone: ++ 49/30/4 50 55 41 31
Fax: ++ 49/30/4 50 55 49 00
Email: Uwe.pleyer@charite.de
